ZE5 Cell Analyzer (Flow Cytometer)
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ZE5 Cell Analyzer (Flow Cytometer)
The ZE5 Cell Analyzer is an innovative flow cytometer with flexible configurations to meet a broad range of experimental complexities and throughput needs – accessible for novice flow cytometry users yet flexible enough for the most experienced flow cytometry professionals.
Product Features
High-Throughput Production
- Detect up to 100,000 events per second without compromising data quality
- Low sample carryover with built-in auto probe wash
- Integrated 96/384/deep-well plate sample loader
Smart Sample-Handling
- Temperature control and vortexing to maintain cell viability and prevent clogging
- Innovative sample pump that takes up samples with minimal dead volume and returns unused sample
Multi-Parameter Analysis
- Up to five lasers and 30 detectors including seven colors off UV for building larger panels, or panels with minimal compensation
- Small particle detector in 3-5 laser configuration for studying exosomes and other small particles
Automation/Robotics-Friendly
- API to connect to LIMS and scheduling software for integration to existing or new workcells
Specifications
Fluorescence sensitivity
Average MESF: FITC: <70 PE: <50 and APC: <40
Scatter sensitivity
<0.2 μm FSC resolution with small-particle detection module
Sample loading
96-well and 384-well microplates, standard or deep-well
40-tube rack of 5 ml tubes (12 x 75 mm)
24-tube rack of microcentrifuge tubes
STAT tube position for single tube loading
40-tube rack of 5 ml tubes (12 x 75 mm)
24-tube rack of microcentrifuge tubes
STAT tube position for single tube loading
Sample throughput
<15 min for 96-well plate and < 60 min for 384-well plate in high-throughput mode
Excitation sources
Up to five spatially separated lasers. Standard options include:
355 nm, 50 mW
488 nm, 100 mW
640 nm, 100 mW
405 nm, 100 mW
561 nm, 50 mW
355 nm, 50 mW
488 nm, 100 mW
640 nm, 100 mW
405 nm, 100 mW
561 nm, 50 mW
Detection
Up to 30 detectors, including FSC and SSC; optional second FSC detector
Cuvette
Fused silica with 145 x 265 μm channel
Speed
Up to 100,000 events per second with all parameters enabled
Data processing
Simultaneous measured peak, area, and width for every channel
24-bit data for area and height
17-bit data for width with high-resolution linear interpolation at the half height
24-bit data for area and height
17-bit data for width with high-resolution linear interpolation at the half height
Fluidics
Bulk fluids: 4 x 4 L bulk fluid tanks on board for sheath and waste
Onboard additive concentrate and cleaner
Onboard additive concentrate and cleaner
Operational flow rates
0.025–3.5 μl/sec
Dimensions (W x D x H)
74 x 69 x 66 cm (29 x 27 x 26 in)
Weight
110 kg (240 lb) maximum
Data format
Flow Cytometry Standard (FCS) 3.1
